Table 1 Comparison of pfhrp2 genotyping protocol with those of other markers generally used in drug efficacy trial
Protocol difference | pfhrp2 | msp1 + msp2 | Microsatellites |
|---|---|---|---|
Molecular protocol | Single-run PCR or PCR–RFLP | Nested PCR | Nested PCR |
Sequencing | Yes** | No | No |
Restriction enzyme | Yes*** | No | No |
No. of PCR reaction | 1 | 7 | 6* |
Number of primers | 2 | 14 | 10–12* |