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Fig. 2 | Malaria Journal

Fig. 2

From: Functional characterization of 5′ UTR cis-acting sequence elements that modulate translational efficiency in Plasmodium falciparum and humans

Fig. 2

130 nucleotides of the 5′ UTR from a translationally active (PF3D7_1428300) and repressed gene (PF3D7_1411400) were sufficient to drive distinct TE. a The diagramed sequence of the full length 5′ UTRs from active PF3D7_1428300 and repressed PF3D7_1411400. uAUGs are marked by green triangles with the different shades representing the three frames. The numbers between the two construct diagrams mark distance from the protein coding region. b The lengths, uAUG count, uORF count, GC content, and translational efficiency (TE) for the chosen 5′ UTRs and genes obtained from the previously published ribosome profiling and mRNA sequencing [18] with the raw luminescence signal (PF RLU) produced by these 5′ UTRs driving NanoLuc (Promega) expression using P. falciparum in vitro translation. c Log10(luminescence) from NanoLuc produced by 5′ UTRs of decreasing length in P. falciparum lysates (red) and K562 lysates (grey). The different lengths were generated by shorting the 5′ UTRs from the 5′ end. d Sequence comparison of the 130 nucleotides closest to the protein coding region of the 5′ UTRs from PF3D7_1411400 (R[WT]) and PF3D7_1428300 (A[WT]). The four uAUGs in R[WT] are labeled with the green triangles. uAUGs without in-frame stops are followed by a dotted line while uORF forming uAUGs are followed by a solid line with the stop is marked by a vertical line. The four uAUGs are labeled 1-4 based on their distance from the protein coding start site

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