Fig. 4
Effect of equally spaced and out of frame, non-terminated uAUGs or uORFs on TE. a Sequence diagram of the two cassettes inserted into R[Δ1:Δ2:Δ3:Δ4] at 5 different positions. The green arrows mark the uAUGs, the solid line indicates the length of the uORF, and the dotted line marks the sequence downstream of the non-terminated uAUG. b The sequence diagrams to the left represent the 5′ UTRs containing the uORF cassette. To the right the uORF cassette and the non-terminated cassette are presented side by side. The left set is from P. falciparum lysates while the right is from human. Graphed for each is the average and SEM of log2(each experimental value/experimental mean of R[Δ1:Δ2:Δ3:Δ4])