Design and methods for a quasi-experimental pilot study to evaluate the impact of dual active ingredient insecticide-treated nets on malaria burden in five regions in sub-Saharan Africa

Table 4 Entomological methods by country

		Burkina Faso	Northern and Western Mozambique	Nigeria	Rwanda
CDCLT collections	Number of collections per district	• 3 villages with 6 houses each		• 4 villages with 2 houses per village • Paired indoor-outdoor collections	• 9 villages with 5 houses per village
CDCLT collections	Frequency	• Once per week • Two consecutive nights		• Three consecutive nights • Once a month	• Two consecutive nights • Twice per month
HLCs	Number of collections per district	• 3 villages with 2 houses each • Paired indoor-outdoor collections	• 3 villages with 3 houses each • Paired indoor-outdoor collections		• 3 houses per village • Paired indoor-outdoor collections
HLCs	Frequency	• Concurrent with CDCLT collections (different houses)	• Two consecutive nights • Every other month for 24 months		• Concurrent with CDCLT collections (different houses)
Pyrethrum spray catches	Number of collections per district			• 48 houses
Pyrethrum spray catches	Frequency			• Once monthly
Larval sampling	Frequency	• Surveys at least once per year	• Surveys at least once per year (aligned with standard NMCP surveillance)	• Surveys at least once per year	• Surveys at least once per year

CDCLT US Centers for Disease Control and Prevention light trap, HLC human landing collection, LGA local government area

ISSN: 1475-2875