Table 1 Primers and probes sequences used for qPCR assays in this study
Target Gene | Oligo sequence | Fluorophores | TM °C |
|---|---|---|---|
Pspp18S F | GCTCTTTCTTGATTTCTTGGATG | Â | 51.71 |
Pspp18S R | AGCAGGTTAAGATCTCGTTCG | Â | 52.4 |
Pspp18S Cy5 | ATGGCCGTTTTTAGTTCGTG | Cyanine 5 | 52 |
PfvarATS F | CCCATACACAACCAAYTGGA | Â | 51.78 |
PfvarATS R | TTCGCACATATCTCTATGTCTATCT | Â | 52.76 |
PfvarATS FAM | TRTTCCATAAAGGT 5ʹ-3ʹ | Fluorescein | NA |
Pv18S F | ACTAGGCTTTGGATGAAAGATTTTA | Â | 53.23 |
Pspp18S R | AACCCAAAGACTTTGATTTCTCATAA | Â | 51.65 |
Pv18S probe | GAATTTTCTCTTCGGAGTTTAT | Cy5-BHQ2 | 46 |
HsRNaseP F | AGATTTGGACCTGCGAGCG | Â | 53.25 |
HsRNaseP R | GAGCGGCTGTCTCCACAAGT | Â | 55.88 |
HsRNaseP_1 | TTCTGACCTGAAGGCTCTGCGCG | HEX | 60.62 |