Table 4 Performance evaluation of three methods overall and among the study Plasmodium falciparum cases, from December 2019 to March 2020, Ethiopia
Characteristics | Sensitivity (CI 95%) | Specificity (CI 95%) | PPV (CI 95%) | NPV (CI 95%) |
|---|---|---|---|---|
Multiplex real-time PCR vs microscopy | ||||
 Overall | 100 (93–100) | 83.2 (77.6–87.9) | 57.9 (47–68.4) | 100 (98–100) |
 P. falciparum | 100 (89–100) | 83.6 (78- 88) | 45.83 (34–58) | 100 (98.2–100) |
RDT vs microscopy | ||||
 Overall | 98 (89.6–99.9) | 95 (91–97.5) | 82 (70–0.6) | 99.5 (97–100) |
 P. falciparum | 100 (89–100) | 95.4 (91.9–7.7) | 75 (59.7–86.8) | 100 (98.4–100) |
Microscopy vs multiplex real-time PCR | ||||
 Overall | 58 (46.9–68.4) | 100 (98–100) | 100 (93–100) | 83.2 (77.6–87.9) |
 P. falciparum | 45.8 (34–58) | 100 (98.2–100) | 100 (89–100) | 83.6 (78.3–88.1) |
RDT vs multiplex real-time PCR | ||||
 Overall | 67 (56.21–76.7) | 98.9 (96–99.9) | 96.7(88.7–9.6) | 86.2 (80.8–90.6) |
 P. falciparum | 56.9 (44.7–68.6) | 98.5 (95.7–9.7) | 93.2 (81.3–8.6) | 86.3 (81.2–90.5) |