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Fig. 3 | Malaria Journal

Fig. 3

From: Analysis of nucleic acids extracted from rapid diagnostic tests reveals a significant proportion of false positive test results associated with recent malaria treatment

Fig. 3

Amplification rate for three single copy genes and ΔCq values of the qHRP2/3-del assay. Amplification rate (rolling mean) for the genotyping reference genes a pfrnr2e2, b pfmsp1 and c pfmsp2 as a function of the parasite density of the sample. Parasite densities for P. falciparum were estimated based on the varATS target of the PlasQ assay. d The distribution of ΔCq values between pfhrp2 (x-axis) or pfhrp3 (y-axis) and pfrnr2e2. ΔCq thresholds (dashed lines) were set at 2.0 for pfhrp2 and 4.0 for pfhrp3. To show the ΔCq for pfhrp2 for samples with a pfhrp3 deletion, the ΔCq for pfhrp3 was set arbitrarily at 6.5

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