Fig. 1

Survival and parasitaemia in lethal and non-lethal infected mice: Balb/c mice were infected with 5 × 10⁵ P. berghei and P. chabaudi parasitized erythrocytes via intra-peritoneal injection. Malaria parasites infected mice were monitored and parasitaemia was examined. Blood smear were prepared from tail vein at various time points as indicated in the figure and stained with giemsa stains. Parasitaemia was determined by counting the percentage of infected cells per 5000 RBCs per slide. A Parasitaemia of P. berghei (triangle, n  =  9) and P. chabaudi (Square, n  = 9) at different time points. B Parasitaemia of P. yoelii17XL (Py17XL) (triangle, n  = 9) and P. yoelii17XNL (Py17XNL) (Square, n  = 9) at different time points. Statistical significance was determined by Student’s t test. **p  < 0.001. C Survival rate of P. berghei and P. chabaudi infected mice. D Survival rate of P. yoelii 17XL and P. yoelii 17XNL infected mice. Nine mice pooled from three independent experiments consisting of three mice in each group. Immunohistochemistry of haemozoin contents in the spleen of Balb/c mice E infected with P. berghei, P. chabaudi, P. yoelii 17XL and P. yoelii 17XNL at 3, 7 and 10 day post infection. Black arrows indicate the haemozoin content at 100 ×  original magnification