Fig. 2

Immune responses in malaria infection with lethal and non-lethal stain of Plasmodium: mice were infected with 5 × 10⁵ P. berghei, P. chabaudi, Py17XL and Py17XNL -parasitized erythrocytes via intra-peritoneal injection. A Splenocytes from infected and control mice were harvested and were stained with antibodies against CD4, and ICOS on 3rd, 10th-day post infection of P. berghei and P. chabaudi. B Splenocytes from infected and control mice were harvested and were stained with antibodies against CD4, and ICOS on 3rd, 10th-day post infection of P. yoelii 17XL and P. yoelii 17XNL. C Determination of Treg cells in malaria infection. Splenocytes were analyzed for Treg by staining with antibodies against CD4⁺ ICOS⁺ and Foxp3⁺cells by flow cytometry. Splenocytes from Pb, Pc infected and control mice were harvested on 3rd and 10th days post infection. D Expression of Treg cells in P. yoelii 17XL and P. yoelii 17XNL malaria infection. Splenocytes from Py17XL, Py17XNL infected and control mice were harvested on 3rd and 10th days post infection. Data is shown from one of the three independent experiments consisting of three mice in each group