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Table 1 Primers used for deep sequencing of Pfdhfr, Pfdhps and Pfmdr1

From: Effect of three years’ seasonal malaria chemoprevention on molecular markers of resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine and amodiaquine in Ouelessebougou, Mali

Locus, primer name

Sequence

Pfdhfr

 PfdhfrF1

CGATCCGAGCGGTGACACATTTAGAGGTCTAGGAAATAAAGG

 PfdhfrR1

CCTCTCTATGGGCAGTCGGTGATTTCTTCCCATAACTACAACATTTTGT

 PfdhfrF2

CGATCCGAGCGGTGAACAAAATGTTGTAGTTATGGGAAGAA

 PfdhfrR2

CCTCTCTATGGGCAGTCGGTGATTTGATAAACAACGGAACCTCCT

Pfdhps

 PfdhpsF1

CGATCCGAGCGGTGAGATGGAGGTATTTTTGTTGAACC

 PfdhpsR1

CCTCTCTATGGGCAGTCGGTGATATTGGTTTCGCATCACATTT

 PfdhpsF2

CGATCCGAGCGGTGATGCATAAAAGAGGAAATCCACA

 PfdhpsR2

CCTCTCTATGGGCAGTCGGTGATACAACATTTTGATCATTCATGC

Pfmdr1

 MDR1F

CGATCCGAGCGGTGATCAGGAGGAACATTACCTTTTT

 MDR1R

CCTCTCTATGGGCAGTCGGTGATACATAAAGTCAAACGTGCATT

  1. Oligos include template-specific sequence as well as overhang adaptors to allow library preparation of amplicons followed by sequencing on an Ion Torrent platform
  2. Substitutions with frequencies below 1% were considered 0