5WBF: a low-cost and straightforward whole blood filtration method suitable for whole-genome sequencing of Plasmodium falciparum clinical isolates

Table 2 WGS statistics of P. falciparum DNA extracted from mock samples subjected to either no treatment or sWGA or 5WBF

Sample name	% para	Enrichment method	Total reads	P. falciparum-mapped reads (×)	Mean genome coverage (×)	Mean coverage of mt genome (×)	Mean coverage of api genome (×)
M1	1.1	None	14,777,245	3,135,321 (21.22)	5.60	45.39	11.76
M1_WGA	1.1	sWGA	16,002,711	15,347,302 (95.9)	74.41	5.20	8.44
M1-1_5F	1.1	5WBF	19,932,109	19,558,272 (98.12)	104.86	2102.17	216.15
M1-2_5F	1.1	5WBF	16,923,729	16,591,158 (98.03)	89.59	1701.08	183.29
M1-3_5F	1.1	5WBF	17,230,853	17,018,367 (98.77)	90.04	1335.70	150.66
M2_WGA	0.23	sWGA	16,266,710	14,112,713 (86.76)	69.16	1.59	3.57
M2-1_5F	0.23	5WBF	14,905,078	14,350,236 (96.28)	79.71	1468.76	120.40
M2-2_5F	0.23	5WBF	19,310,196	18,169,487 (94.09)	99.82	1782.77	161.75
M2-3_5F	0.23	5WBF	17,672,315	16,766,350 (94.87)	91.15	1933.32	129.47
M3_WGA	0.022	sWGA	9,840,798	4,915,197 (49.95)	18.63	0.31	2.34
M3-1_5F	0.022	5WBF	17,283,136	14,467,900 (83.71)	76.72	1209.77	115.57
M3-2_5F	0.022	5WBF	17,759,091	11,975,821 (67.43)	62.32	1528.46	98.56
M3-3_5F	0.022	5WBF	13,052,530	9,839,100 (75.38)	52.17	1026.41	59.63

ISSN: 1475-2875