Table 1 Primers for PCR genotyping of block 2 region of msp-1
Amplication allele primer | Primer sequence | |
|---|---|---|
Primary PCR | NA | Forward 5’-CTAGAAGCTTTAGAAGATGCAGTATTG-3’ Reverse 3’-CTTAAATAGTATTCTAATTCAAGTGGATCA-5’ |
Secondary PCR | K1 | Forward 5’-AAATGAAGAAGAAATTACTACAAAAGGTGC-3’ Reverse 3’-GCTTGCATCAGCTGGAGGGCTTGCACCAGA-5’ |
MAD20 | Forward 5’-AAATGAAGGAACAAGTGGAACAGCTGTTAC-3’ Reverse 3’-ATCTGAAGGATTTGTACGTCTTGAATTACC-5’ | |
RO33 | Forward 5’-TAAAGGATGGAGCAAATACTCAAGTTGTTG-3’ Reverse 3’-CATCTGAAGGATTTGCAGCACCTGGAGATC-5’ | |