Durability of three types of dual active ingredient long-lasting insecticidal net compared to a pyrethroid-only LLIN in Tanzania: methodology for a prospective cohort study nested in a cluster randomized controlled trial

Martin, Jackline L.; Messenger, Louisa A.; Mosha, Franklin W.; Lukole, Eliud; Mosha, Jacklin F.; Kulkarni, Manisha; Churcher, Thomas S.; Sherrard-Smith, Ellie; Manjurano, Alphaxard; Protopopoff, Natacha; Rowland, Mark

doi:10.1186/s12936-022-04119-4

Table 1 Summary of the bioassay testing plan and outcomes per net type and mosquito strain

From: Durability of three types of dual active ingredient long-lasting insecticidal net compared to a pyrethroid-only LLIN in Tanzania: methodology for a prospective cohort study nested in a cluster randomized controlled trial

		Interceptor G2^®	Royal Guard^®	Olyset™ Plus
Susceptible strain: assessment of pyrethroid
	Treatment	1/Untreated, 2/Interceptor, 3/Interceptor G2^®	1/Untreated, 2/Interceptor, 3/Royal Guard^®	1/Untreated, 2/Interceptor, 3/Olyset ™ Plus
Cone	Outcomes	Kd^a, mortality 24, 48 and 72 h^b	Kd, mortality 24, 48 and 72 h	Kd, mortality 24, 48 and 72 h
	Exposure time	3 min	3 min	3 min
	Total nets	30 nets (t0 to t30), 50 nets (t36)	30 nets (t0 to t30), 50 nets (t36)	30 nets (t0 to t30), 50 nets (t36)
	Total pieces	5 (t0) and 4 subsequent follow ups (position 2 to 5)	5 (t0) and 4 subsequent follow ups (position 2 to 5)	5 (t0) and 4 subsequent follow ups (position 2 to 5)
	Total replicates/piece	4	4	4
	Total Mosquito per test	5	5	5
	Total mosquitoes	Total between 4800 to 6000 per time point	Total between 4800 to 6000 per time point	Total between 4800 to 6000 per time point
Tunnel	Outcomes	Kd, mortality 24, 48 and 72 h, blood feeding	Kd, mortality 24, 48 and 72 h, blood feeding	Kd, mortality 24, 48 and 72 h, blood feeding
	Exposure time	12 to 15 h	12 to 15 h	12 to 15 h
	Total nets	All failed net with cone	All failed net with cone	All failed net with cone
	Total pieces	1 per net	1 per net	1 per net
	Total replicates/piece	2 replicates	2 replicates	2 replicates
	Total Mosquito per test	50	50	50
	Total mosquitoes	Determined by number of failing nets	Determined by number of failing nets	Determined by number of failing nets
Resistant strain: assessment of partner A.I. or synergist
Cone	Outcomes		Kd, mortality 24, 48 and 72 h, ovarial development	Kd, mortality 24, 48 and 72 h
	Exposure time		3 min	3 min
	Total nets		30 nets (t0 to t30), 50 nets (t36)	30 nets (t0 to t30), 50 nets (t36)
	Total pieces		5 (t0) and 4 (t6 to t36)	5 (t0) and 4 (t6 to t36)
	Total replicates/piece		4	4
	Total Mosquito per test		5	5
	Total mosquitoes		Total between 4800 to 6000 per time point	Total between 4800 to 6000 per time point
Tunnel	Outcomes	Kd, mortality 24, 48 and 72 h, blood feeding	Kd, mortality 24, 48 and 72 h, Blood feeding, ovarial development	Kd, mortality 24, 48 and 72 h, blood feeding
	Exposure time	12 to 15 h	12 to 15 h	12 to 15 h
	Total nets	30 nets (t0 to t30), 50 nets (t36)	All failed net with cone	All failed net with cone
	Total pieces	1 per net (position 2)	1 per net	1 per net
	Total replicates/piece	2 replicates	2 replicates	2 replicates
	Total Mosquito per test	50	50	50
	Total mosquitoes	30 (50) nets × 1-piece × 2 replicates × 50 mosquitoes × 3 treatments = 9000	Determined by number of failing nets	Determined by number of failing nets
	Net Specificity	slow killing effect 72 h; mortality main outcome	Ovarial development by dissection at 72 h post exposure	Colony mosquito’s resistance strain (kdr^c and kdr + MFO^d)

^aKnockdown
^bHours
^cKnockdown resistance
^dCytochrome P450 mono-oxygenase mechanisms

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ISSN: 1475-2875

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