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Table 1 Genetic diversity indices and neutrality tests (Fu’s Fs and Tajima’s D) based on the COII gene of An. nivipes

From: Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border

Species

n

Haplotype code

S

Pi

h

Hd

k

Fu's Fs

Tajima's D

Total

86

H1(19), H2(13), H3(1), H4(1), H5(1), H6(8), H7(1), H8(1), H9(1), H10(1), H11(1), H12(2), H13(1), H14(1), H15(1), H16(1), H17(1), H18(1), H19(1), H20(1), H21(1), H22(1), H23(1), H24(1), H25(1), H26(1), H27(1), H28(2), H29(1), H30(1), H31(1), H32(1), H33(1), H34(1), H35(1), H36(1), H37(1), H38(1), H39(1), H40(1), H41(1), H42(1), H43(1), H44(1), H45(1), H46(1), H47(1)

223

0.01560

47

0.92370

8.12558

− 19.201***

− 2.39879***

KH_St_Sp

53

H1(19), H2(12), H3(1), H4(1), H5(1), H6(8), H7(1), H8(1), H9(1), H10(1), H11(1), H12(2), H13(1), H14(1), H15(1), H16(1)

19

0.00243

16

0.80700

1.50363

− 10.944***

− 2.01237**

IN_Tri

4

H24(1), H25(1), H26(1), H27(1)

8

0.00701

4

1.00000

4.33333

− 0.715

− 0.06867

IN_Pun_Ch

2

H21(1), H22(1)

13

0.01268

2

1.00000

7.00000

1.946

n.d

IN_Pun_Ba

2

H20(1), H23(1)

65

0.04415

2

1.00000

23.00000

3.135

n.d

IN_Nag

6

H36(1), H37(1), H38(1), H39(1), H40(1), H41(1)

14

0.01079

6

1.00000

6.66667

− 1.623

0.53608

IN_Ass

7

H33(1), H34(1), H35(1), H44(1), H45(1), H46(1), H47(1)

28

0.02350

7

1.00000

14.52381

− 0.938

0.09554

MM

2

H17(1), H18(1)

2

0.00324

2

1.00000

2.00000

0.693

n.d

TH

2

H2(1), H19(1)

3

0.00485

2

1.00000

3.00000

1.099***

n.d

IN_Miz

2

H42(1), H43(1)

3

0.00485

2

1.00000

3.00000

1.099***

n.d

IN_Meg

6

H28(2), H29(1), H30(1), H31(1), H32(1)

11

0.00744

5

0.93330

4.60000

− 0.496

− 0.27307

  1. n.d., not determined; n.s., P > 0.10; #P < 0.10; *P < 0.05; **P < 0.02; ***P < 0.001. KH_St_Sp, Siem Pang County (Stung treng, Cambodia); IN_Tri, Tripura (India); IN_Pun_Ch, Cheema (Punjab, India); IN_Pun_Ba, Bathinda (Punjab, India); IN_Nag, Nagaland (India); IN_Ass, Assam (India); MM, Myanmar; TH, Thailand; IN_Miz, Mizoram (India); IN-Meg, Meghalaya (India)
  2. n number of sequences, S number of polymorphic sites, pi nucleotide diversity, h number of haplotypes, Hd haplotype diversity