DNA isolation method | Sample input | Lysis time | Elution volume | Dilution factor after DNA elution* |
---|---|---|---|---|
NucleoMag Blood 200 μL Kit | ||||
50 μL whole blood | 10 min | 50 μL | 1 × | |
50 μL DBS | 90 min | 50 μL | 1× | |
50 μL DBS (short) | 40 min | 50 μL | 1× | |
5 × 3 mm punches DBS | 90 min | 50 μL | 3.33× | |
QIAamp DNA Blood Mini Kit | ||||
50 μL whole blood | 10 min | 50 μL | 1× | |
50 μL DBS | 90 min | 50 μL | 1× | |
5 × 3 mm punches DBS | 90 min | 50 μL | 3.33× | |
Tween-Chelex | ||||
50 μL DBS | Overnight | ~ 250 μL | ~ 5× | |
5 × 3 mm punches DBS | Overnight | ~ 70 μL | ~ 4.66× | |
Direct PCR | ||||
15 μL whole blood | NA | NA | NA | |
5 × 3 mm punches DBS | NA | NA | NA |