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Table 1 Overview of the different DNA isolation methods and conditions

From: Tenfold difference in DNA recovery rate: systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance

DNA isolation method

Sample input

Lysis time

Elution volume

Dilution factor after DNA elution*

NucleoMag Blood 200 μL Kit

 

50 μL whole blood

10 min

50 μL

1 × 

 

50 μL DBS

90 min

50 μL

 

50 μL DBS (short)

40 min

50 μL

 

5 × 3 mm punches DBS

90 min

50 μL

3.33×

QIAamp DNA Blood Mini Kit

 

50 μL whole blood

10 min

50 μL

 

50 μL DBS

90 min

50 μL

 

5 × 3 mm punches DBS

90 min

50 μL

3.33×

Tween-Chelex

 

50 μL DBS

Overnight

 ~ 250 μL

 ~ 5×

 

5 × 3 mm punches DBS

Overnight

 ~ 70 μL

 ~ 4.66×

Direct PCR

 

15 μL whole blood

NA

NA

NA

 

5 × 3 mm punches DBS

NA

NA

NA

  1. *As determined by sample DNA input versus elution volume (e.g., 50 μL whole blood eluted in 50 μL results in a dilution factor of 1x)