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Table 4 DNA quality by A260/A280 absorbance ratios of different DNA extraction methods

From: Tenfold difference in DNA recovery rate: systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance

DNA isolation method

Sample input

Mean A260/A280 ratio (± SD)

NucleoMag Blood 200 μL Kit

 

50 μL whole blood

1.86 (± 0.06)

 

50 μL DBS

1.83 (± 0.04)

 

50 μL DBS (short lysis)

1.86 (± 0.12)

 

5 × 3 mm punches DBS

1.88 (± 0.15)

QIAamp DNA Blood Mini Kit

 

50 μL whole blood

1.67 (± 0.08)

 

50 μL DBS

1.62 (± 0.17)

 

5 × 3 mm punches DBS

1.66 (± 0.05)

Tween-Chelex

 

50 μL DBS

1.16 (± 0.02)

 

5 × 3 mm punches DBS

1.31 (± 0.07)

Direct PCR

 

15 μL DBS

1.70 (± 0.02)

 

5 × 3 mm punches DBS

1.63 (± 0.03)

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Malaria Journal

ISSN: 1475-2875

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