Fig. 3
From: The ecdysone receptor regulates several key physiological factors in Anopheles funestus
Several reproductive factors are affected when EcR is depleted. The median number of eggs oviposited did not differ between dsEcR and dsGFP (p > 0.05). A The total number of eggs developed in mated females was significantly decreased in dsEcR injected An. funestus females compared to dsGFP An. funestus females (p < 0.0001). B The development of eggs differed between mated dsGFP controls and mated dsEcR treated An. funestus females. C Mature, developed eggs (ovoid shape) were observed in mated uninjected and dsGFP treated An. funestus females. Contrastingly, dsEcR treated An. funestus females contained immature and undeveloped oocytes (spheroid shaped) in their ovaries that more closely resembled the ovaries of virgin An. funestus females of all treatment groups. The median fertility of dsEcR treated An. funestus females was significantly lower compared to dsGFP An. funestus females (p <  0.01). D Error bars represent SEM. ** denotes p < 0.05; **** denotes p < 0.0001; ns = not statistically significant (p > 0.05). n represents sample size