Fig. 3

GMZ2-specific B cell frequencies following immunization. B cells were estimated using cryopreserved PBMCs without additional stimulation. The comparison of the GMZ2-specific memory B cells frequencies between D0 and D84 was performed in those receiving the control vaccine and in those vaccinated with GMZ2 (including those vaccinated with 100 µg GMZ2-Alhydrogel (opened dots), 30 µg GMZ2-CAF01 (grey dots), 100 µg GMZ2-CAF01 (black dots) using Wilcoxon test with Bonferroni correction for multiple comparisons. p value less than 0.05 is considered as statistically significant. All time points per volunteer were measured in a single experiment after several optimization tests, and individual volunteers were measured in separate experiments. Symbols represent individual samples. Red lines represent median values with interquartile range