A reverse vaccinology approach on transmembrane carbonic anhydrases from Plasmodium species as vaccine candidates for malaria prevention

Table 1 Prediction of binding affinity and MHC-peptide complex stability for α- and η-CA protein sequences from Plasmodium spp.

CA class	HLA-A and -B alleles	Peptide sequence	NetMHCpan Affinity^a (0 ≤ X ≤ 1)	NetMHCstab Stability (hours)	Antigenicity prediction	Result^b
α-CA (group 1) (W7JAI7)	HLA-A*24	509-IYFILFIFYNIVLF-522	0.913	15.14	501-FSYYSKWDIYFILFIFYNIVL-521 (Transmembrane sequence)	HS
	HLA-A*11	399-STLPLCDENVSWK-411	0.899	13.09	398-SSTLPLCDEN-407	HS
	HLA-B*53	469-FPIQVLISSAI-479	0.717	–	461-RKFSLVQVFPIQVLISSAIS-480	SB
α-CA (group 2) (Q8IHW5)	HLA-A*24	585-IYFILFIFYNIVLF-598	0.913	15.14	582-KWDIYFILFIFYNIVL-597 (Transmembrane sequence)	HS
	HLA-A*11	476-STLPLCDENVSWK-488	0.899	13.09	475-SSTLPLCDEN-484	HS
	HLA-B*53	8-YPILLFYNVNVF-19	0.695	–	4-LYLLYPILLFYNVNVFINY-22	SB
η-CA (V7PFH4)	HLA-A*02	633-YLIQGFPV-640	0.925	17.67	633-YLIQGFPV-640	HS
η-CA (V7PFH4)	HLA-B*53	638-FPVQLLISSAL-648	0.638	–	629-YGRVYLIQGFPVQLLISSALT-649	SB

HS highly stable, SB strong binding
Transmembrane domains of α-CAs (W7JAI7 and Q8IHW5) from P. falciparum, which are suggested to be buried from the access of MHC-I and MHC-II systems
^aThreshold for SB is 0.5%
^bThe threshold stability for HS and SB is 6 h. Strong binding peptides (IC₅₀ ≤ 50 nM or %-Rank ≤ 0.5)

ISSN: 1475-2875