Fig. 4

Effect of serum concentration on the ability of the rLDH7-based APTEC µPAD platform to detect the presence of 133 nM rPfLDH spiked into the sample. A Colour-enhanced photographs of the colorimetric response of the rLDH7 µPAD in the presence of 133 nM rPfLDH prepared in varying concentrations of serum. B Scatterplot comparing the influence of serum concentration on resultant colour intensities in the sensors presented in (A). ANOVA results are presented as annotations inset within the graph. *- indicates serum concentrations that resulted in Zone 1 colorimetric responses significantly lower to those found in 0.1% serum samples (p < 0.05; Tukey’s post hoc test). C Scatterplot comparing the contrast (ΔI of test zones – ΔI of control zone 2) for individual sensors. ANOVA results are presented as annotations inset within the graph. †—indicates p ≤ 0.05 for a 1-sample t test for Zone 1 average contrasts, testing againt the null hypothesis that the average = 0