Skip to main content
Fig. 2 | Malaria Journal

Fig. 2

From: Expression and copper binding characteristics of Plasmodium falciparum cytochrome c oxidase assembly factor 11, Cox11

Fig. 2

Evaluation of rMBPPfCox11Ct on SDS-PAGE and detection with anti-MBP antibodies on a western blot. Proteins were separated on a 12.5% reducing SDS-PAGE gel and stained with Coomassie blue (A) and (B) the same proteins on an identical gel electrophoretically transferred to nitrocellulose and probed with mouse anti-MBP monoclonal antibody and detected with goat anti-mouse IgG-horse radish peroxidase (HRPO) conjugate and enhanced chemiluminescence (ECL). Mw: molecular weight marker; lane 1, soluble E. coli lysate, and fractions from an amylose affinity matrix: unbound (lane 2); wash (Lane 3) and eluted in the presence of maltose (lanes 4–8)

Back to article page