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Fig. 5 | Malaria Journal

Fig. 5

From: Expression and copper binding characteristics of Plasmodium falciparum cytochrome c oxidase assembly factor 11, Cox11

Fig. 5

Copper binding to rMBPPfCox11Ct and mutants in vivo measured by the BCA release assay. E. coli host cells expressing rMBPPfCox11Ct or the mutants C60A, C157A and C60A/C157 or rMPB were grown in the presence of 0.5 mM CuCl2. The expressed protein was affinity purified and copper was detected with the BCA release assay without (open bars) or with (closed bars) the addition of ascorbic acid. Results are the means ± SE of triplicate measurements from duplicate samples. A two-way ANOVA with Bonferroni multiple comparison tests were conducted on the results comparing: rMBPPfCox11Ct and mutants wrt rMPB *P < 0.05 and **P < 0.001, rMBPPfCox11Ct wrt C157A and C60A/157A ##P < 0.001, C157A wrt C60A/C157A $$P < 0.001

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Malaria Journal

ISSN: 1475-2875

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