Fig. 9

Detection of Plasmodium spp. Cox11 and rMBPPfCox11Ct with chicken anti-rMBPPfCox11Ct IgY and chicken anti-KIQF{Abu}F{Abu}EEQMLNAKEEM peptide IgY. A 12.5% SDS-PAGE reducing gel stained with Coomassie blue (A, C) and the same proteins on an identical gel electrophoretically transferred to nitrocellulose (B, D) and probed with B chicken anti-rMBPPfCox11-Ct IgY or D chicken anti-KIQF{Abu}F{Abu}EEQMLNAKEEM peptide IgY. A–D Mw molecular weight markers; lane 1, uninfected red blood cell lysate; lane 2, untransformed E. coli BL21(DE3) host cell lysate; lane 3, E. coli expressing rMBPPfCox11Ct; lane 4, affinity purified rMBPPfCox11Ct and lane 5, affinity purified recombinant rMPB. E Coomassie Blue stained reference gel and proteins transferred to nitrocellulose and probed with F chicken anti-Plasmodium lactate dehydrogenase peptide IgY and G chicken anti-rMBPPfCox11Ct IgY. E–G Mw molecular mass marker (Mw); lysates of uninfected (lane 1) and (lane 2) P. berghei infected BALB/c mouse red blood cells. Chicken IgY antibodies were detected with rabbit anti-IgY-HRPO secondary antibody and ECL