Table 1 Accuracy and precision for the quantification of ( ±)-primaquine and ( ±)-carboxyprimaquine in human EDTA plasma
Analyte | Sample | Nominal concentration (ng/mL) | Measured concentration (ng/mL) | Accuracy (%) | Between-assay precision (RSD) | Within-assay precision (RSD) |
|---|---|---|---|---|---|---|
( +)-PRQ | LLOQ | 0.571 | 0.591 | 103 | 7.72 | 9.93 |
QC1 | 1.46 | 1.48 | 101 | 6.38 | 6.32 | |
QC2 | 16.8 | 16.8 | 100 | 6.27 | 3.60 | |
QC3 | 195 | 193 | 98.9 | 3.08 | 3.54 | |
ULOQ | 260 | 256 | 98.5 | 6.77 | 4.00 | |
Over curve | 519 | 515 | 99.2 | 4.20 | 2.81 | |
(−)-PRQ | LLOQ | 0.571 | 0.556 | 97.4 | 13.3 | 9.08 |
QC1 | 1.46 | 1.43 | 97.9 | 7.12 | 4.54 | |
QC2 | 16.8 | 17.1 | 101 | 6.67 | 1.13 | |
QC3 | 195 | 192 | 98.5 | 1.45 | 1.03 | |
ULOQ | 260 | 252 | 96.9 | 2.80 | 1.05 | |
Over curve | 519 | 530 | 102 | 1.62 | 1.64 | |
( +)-CPRQ | LLOQ | 2.44 | 2.39 | 97.9 | 8.12 | 4.93 |
QC1 | 7.32 | 7.28 | 99.5 | 8.84 | 9.13 | |
QC2 | 117 | 116 | 99.1 | 3.34 | 2.50 | |
QC3 | 1875 | 1806 | 96.3 | 7.61 | 2.19 | |
ULOQ | 2500 | 2471 | 98.8 | 9.31 | 2.39 | |
Over curve | 5000 | 4886 | 99.7 | 7.45 | 3.87 | |
(−)-CPRQ | LLOQ | 2.44 | 2.31 | 94.7 | 5.90 | 8.23 |
QC1 | 7.32 | 7.22 | 98.6 | 11.2 | 7.45 | |
QC2 | 117 | 114 | 97.4 | 5.18 | 1.68 | |
QC3 | 1875 | 1813 | 96.7 | 4.70 | 4.03 | |
ULOQ | 2500 | 2404 | 96.2 | 2.80 | 1.05 | |
Over curve | 5000 | 4938 | 98.8 | 1.62 | 1.64 |