Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

Malaria Journal

Table 1 List of spiked samples in the blood and DBS panels used in this study

Category	Samples	Source	Type	Dilution	Matrix	# of samples
International standard	WHO international standard P. falciparum antigen	NIBSC	Cell culture	9-points + 1 blank	WB, DBS	10
International standard	WHO international standard P. vivax antigen	NIBSC	Pool of clinical	9-points + 1 blank	WB, DBS	10
Detection of failed HRP2/HRP3 expression	P. falciparum, W2 (hrp2 + hrp3 +)	PATH	Cell culture	5-points	WB, DBS	5
	P. falciparum, Dd2 (hrp2–hrp3 +)	PATH	Cell culture	5-points	WB, DBS	5
	P. falciparum, 3DB5 (hrp2–hrp3–)	PATH	Cell culture	5-points	WB, DBS	5
Reactivity to native proteins	P. falciparum	Discovery life science	Clinical	5-points	WB, DBS	15
	P. vivax	Discovery life science	Clinical	5-points	WB, DBS	10
	P. malariae	Discovery life science	Clinical	5-points	WB, DBS	10
	P. knowlesi (A1-H1)	PATH	Cell culture	5-points	WB, DBS	5
Baseline specificity	Negative (a pool of 5)		NA	NA	WB, DBS	2
Total						77

ISSN: 1475-2875