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Table 2 Inter-assay variability for malaria antigen signal intensity determined using Q-Plex and xMAP platforms

From: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

Sample type

Analyte

International standard for Plasmodium antigen, CV (%)

Q-Plex

xMAP

50 IU/mL

25 IU/mL

12.5 IU/mL

6.3 IU/mL

Average

50 IU/mL

25 IU/mL

12.5 IU/mL

6.3 IU/mL

Average

Whole blood

HRP2

1.2

4.8

3.7

9.6

4.8

2.9

4.9

1.5

2.3

2.9

PanLDH

6.0

3.8

6.3

7.8

6.0

3.9

1.1

10.9

2.7

4.6

PfLDH

3.3

2.9

0.3

8.1

3.7

8.9

10.0

10.2

5.4

8.6

PvLDH

0.9

2.1

2.0

2.2

1.7

8.4

10.2

12.9

9.7

9.4

DBS

HRP2

4.9

3.1

2.6

2.9

3.4

6.6

2.5

1.9

2.9

3.5

PanLDH

34.3

32.6

24.9

13.6

26.3

7.0

4.7

4.5

9.0

6.3

PfLDH

8.2

10.1

7.3

7.5

8.3

10.8

2.7

1.9

0.8

4.1

PvLDH

25.6

10.0

16.3

38.4

24.6

12.1

7.7

8.0

6.7

8.8

  1. Samples were tested on multiple days by one operator. Assays for HRP2, PanLDH, and PfLDH were assessed using the WHO international standard P. falciparum antigen, while the PvLDH assay was assessed using the WHO international standard P. vivax antigen
  2. International standard samples (n = 3) at different concentrations
  3. CV coefficient of variation, IU international unit