Skip to main content

Table 3 Identification of P. falciparum mutants with hrp2/hrp3 deletions

From: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

Sample type

P. falciparum strain

Number of samples

% Positive agreement

Q-Plex

xMAP

Wild type

hrp2/hrp3 deletions

Wild type

hrp2/hrp3 deletions

Whole blood

W2 (hrp2+hrp3+)

5

100

NA

100

NA

Dd2 (hrp2–hrp3+)

5

100

NA

100

NA

3BD5 (hrp2–hrp3–)

5

100

100

100

100

DBS

W2 (hrp2+hrp3+)

5

100

NA

100

NA

Dd2 (hrp2–hrp3+)

5

80

NA

100

NA

3BD5 (hrp2–hrp3–)

5

40

40

80

80

  1. The established cutoff values were used to determine whether a sample tests positive and negative for HRP2 and PfLDH antigens. P. falciparum infection was determined by the test results with the presence of HRP2 or/and PfLDH above the cutoff. The hrp2/hrp3 deletions were identified by the test results that showed no detectable HRP2 in the presence of PfLDH above the cutoff. The cutoff was determined by the concentration yielding 99.5% or more diagnostic specificity for the Q-Plex and the mean of negative controls + 3 standard deviation for the xMAP
  2. NA not applicable