Maintenance of high temporal Plasmodium falciparum genetic diversity and complexity of infection in asymptomatic and symptomatic infections in Kilifi, Kenya from 2007 to 2018

Table 1 Characteristics of the cohort and number of samples successfully genotyped from 2007 to 2018

Year	Microscopy positivity rate* (%)	Median age in years	Asymptomatic episode		First febrile episode
Year	Microscopy positivity rate* (%)	Median age in years	n	Samples available [% genotyped]	n	Samples available [% genotyped]
2007	16.2	4.9	85	55 [80.0]	18	0
2008	22.1	5.4	122	110 [59.1]	33	15 [6.7]
2009	19.9	6.1	119	96 [46.9]	34	20 [95.0]
2010	26.8	6.9	237	223 [22.4]	115	39 [64.1]
2011	22.6	7.7	146	62 [77.4]	47	28 [64.3]
2012	17.4	7.8	179	68 [61.8]	16	4 [25.0]
2013	9.0	8.1	105	52 [69.2]	32	7 [100.0]
2014	14.2	8.7	165	41 [0]	73	15 [46.7]
2015	17.4	8.2	98	60 [73.3]	27	5 [100.0]
2016	11.7	7.5	73	36 [58.3]	13	4 [100.0]
2017	4.3	6.7	14	13 [76.9]	4	4 [75.0]
2018	5.5	6.8	16	22 [27.3]	7	6 [33.3]

NB: n refers to number of individuals in the cohort each year defined as asymptomatic and first-febrile during follow up visits. In 2007, there were no corresponding febrile samples in the biobank and in 2014 asymptomatic samples failed PCR amplification. Positivity rate was determined by microscopy, *there was a significant decline (Mann–Kendall trend analysis p < 0.001). % genotyped is the percentage of PCR amplified amplicons that yielded successful fragments

ISSN: 1475-2875