Table 1 The mix of primers and probes used in this study,
Species | Oligo name | Target region | Oligo final concentrations (TaqMan assay) (µM) | Oligo final concentrations (HRM assay) (µM) | Supplier |
|---|---|---|---|---|---|
Pan-Plasmodium | Pspp18S fwd | 18S rRNA | 0.4 | 0.3 | Eurogentec |
Pspp18S rev | 18S rRNA | 0.4 | 0.3 | Eurogentec | |
Pspp18S probe | 18S rRNA | 0.2 | – | Eurogentec | |
P. falciparum | PfvarATS fwd | varATS | 0.4 | 0.3 | Eurogentec |
PfvarATS rev | varATS | 0.4 | 0.3 | Eurogentec | |
PfvarATS probe | varATS | 0.25 | – | Eurogentec | |
H. sapiens (Ci) | HsRNaseP fwd | Rnasep gene | 0.2 | – | Eurogentec |
HsRNaseP rev | Rnasep gene | 0.2 | – | Eurogentec | |
HsRNaseP probe | Rnasep gene | 0.1 | – | Eurogentec |