Table 3 Sensitivity and Specificity of microscopy and the different qPCR methods studied compared to LAMP Alethia® Malaria
Microscopy | Plasmodium Typage (Bio-Evolution) | Ampliquick® Malaria | In-house HRM | In-house TaqMan | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
+ | − | + | − | + | − | + | − | + | − | ||||
LAMP + (N = 104) | 86 | 18 | 99 | 5 | 101 | 3 | 102 | 2 | 104 | 0 | |||
LAMP − (N = 43) | 0 | 43 | 0 | 43 | 0 | 43 | 0 | 43 | 0 | 43 | |||
Specificity | 100% | 100% | 100% | 100% | 100% | ||||||||
Sensitivity 95% CI | 85.2% [74–89.4] | 95.4% [89.1–98.4] | 97.2% [91.8–99.4] | 98.1% [93–99.8] | 100%[100–100] | ||||||||
Kappa 95% CI | 0.74 [0.63–0.85] | 0.92 [0.85–0.99] | 0.95 [0.9–1] | 0.97 [0.92–1] | 1 [1–1] | ||||||||