Table 2 qPCR assays: primer positions, parasite reference target, human gene and amplicon size
qPCR assay initial report (reference) | pfhrp2/3 target position | Parasite reference gene | Internal control | Amplicon size (base pairs) |
|---|---|---|---|---|
Grignard et al. [37] | Exon 2 | pfldh | Human TUBB | pfhrp2: 98 pfhrp3: 84 |
Kreidenweiss et al. [39] | Exon 2 | Parasite pfbtub | None | pfhrp2: 78 pfhrp3: 79 |
Schindler et al. [38] | Exon 1–2 | pfrnr2e2 | None | pfhrp2: 286 pfhrp3: 289 |