DNA recovery from used malaria RDT to detect Plasmodium species and to assess Plasmodium falciparum genetic diversity: a pilot study in Madagascar

Table 1 Primary and secondary PCR primers

Gene	Allelic type	PCR round	Primer sequence (5′–3′)	Fragment size (bp)
Msp1		Primary PCR	CACATGAAAGTTATCAAGAACTTGTC	633
		Primary PCR	GTACCGCTAATTCATATTCTATTGCTAG	633
	MAD20	Nested PCR	GAACAAGTSGAACAGCTGTTA	120–250
	MAD20	Nested PCR	TGAATTATCTGAAGGATTTGTACGCCT	120–250
	K1	Nested PCR	GAAATTACTACAAAAGGTGCCAAGTG	160–300
	K1	Nested PCR	AGATGAAGTATTTGAACGAGCTAAAGT	160–300
	RO33	Nested PCR	GCAAATACTCAAGTTGTTGCAAAGC	100–160
	RO33	Nested PCR	AGGATTTGCAGCAYCCTGGAGATCT	100–160
Msp2		Primary PCR	ATGAAGGTAATTAAAACATTGTCTATTAAT	811
		Primary PCR	ATATGGCAAAAGATAAAACAAGTGTTGCTG	811
	3D7	Nested PCR	GCAGAAAGTAAKGCCTYTCTACTGGTGCT	150–350
	3D7	Nested PCR	AGATGAAGTATTTGAACGAGGTAAAGTG	150–350
	FC27	Nested PCR	GCAAATGAAGGTTCTAATACTAATAG	300–600
	FC27	Nested PCR	GCTTTGGGTCCTTCTTCAGTTGATTC	300–600
Glurp		Primary PCR	ATG AAT TYG AAG ATG TTC ACA CTG AAC	1200
		Primary PCR	ATG AAT TYG AAG ATG TTC ACA CTG AAC	1200
		Nested PCR	CTG AAC CAA ATCA AAA TAA CG	600–1000
		Nested PCR	TTC TTC TGG TTT TAT AGT TTC	600–1000

ISSN: 1475-2875