Fig. 1

A Restrained blood feeding of female Culex quinquefasciatus on a domestic canary. Athletic bandage was used as a restraint. Canaries were restrained for up to 30 min. B Mosquitoes were kept alive for 10 days in a glass tube placed into the Drosophila Locomotor Activity Monitor 25. On one end, plastic test-tubes were used to hold the 10% sucrose solution and a sugar wick. The tubes were held in place with multiple layers of parafilm for a tight fit. On the other end, a cotton ball was used to keep the mosquito within the glass tube. Careful attention was paid to prevent the wick and cotton ball from extending too far into the glass tube so that erroneous recordings were not collected