Fig. 2

An experimental timeline of activities during experimentation. The top line refers to the post-infection days of Cx. quinquefasciatus with P. relictum (18 days total). The bottom line refers to the days that mosquito activity was being recorded in the environmental chamber (10 days total). Gray shaded days were removed from analyses, and green shaded days were included. Letters represent points of interest as follows. A Mosquitoes were infected with P. relictum, B mosquitoes that laid eggs were added to the activity monitor, C all mosquitoes were removed from the activity monitor and dissected, D the midpoint separating early and late day analyses relating to the timing of sporozoite entry into the mosquito salivary glands. Day 12 post-infection corresponds with when it is expected to see sporozoite presence in mosquito salivary glands