Fig. 1

Principal component analysis (PCA) of transcriptome variation in Culex quinquefasciatus mosquitoes at three time point after feeding on either a negative-control canary (Uninfected) or a canary infected with a Hawaiian strain of Plasmodium relictum GRW4 (Infected). Time points analysed were: 24 h post feeding (24 hpf = ookinete invasion of mosquito midgut), 5 days post feeding (5 dpf = oocyst development), and 10 days post feeding (10 dpf = oocyst maturation, sporozoite release and invasion of salivary glands). Most of the variance was explained by mosquitoes 24 hpf regardless of Plasmodium infection status