Table 2 Nested 1 PCR protocol
Nested 1 PCR | Primers: PLU3 & PLU4 | |
|---|---|---|
Step | Temperature (°C) | Time |
Initialization | 94 | 5Â min |
55 | 30Â s | |
72 | 2Â min | |
Replication (29 cycles) | ||
 Denaturation | 94 | 30 s |
 Annealing | 55 | 30 s |
 Extension | 72 | 1 min 30 s |
Final extension | 72 °C | 5 min |