Fig. 4

Parasite morphology in control (mock treatment; + 0.1% DMSO) or drug treated (+ 20 nM DHA and + 700 nM DHA) cultures. A Representative images from Giemsa-stained thin smears showing morphology of parasites at 72 h of treatment. Most of the 3D7, F^MT and A^MT parasites treated with DHA were identified as pyknotic and dead (indicated by arrows). In contrast, a marked proportion of K^MT, K^MTF^MT, and K^MTF^MTA^MT parasites appeared viable and mostly at the late trophozoite and early schizont stages of development. MRA1240 parasites also appeared viable and mostly at the ring stage, with some early trophozoites, consistent with some growth delay. B The percent infected red cells containing trophozoites and schizonts (blue), rings (red) and pyknotic forms (green)