Significance of nested PCR testing for the detection of low-density malaria infection amongst febrile patients from the Malaria Elimination Demonstration Project in Mandla, Madhya Pradesh, India

Table 1 Primer sequence and PCR condition used for amplification of Plasmodium species

Genus/species	Primer name	Primer sequence	PCR product length (bp)	Denaturation	Annealing	Elongation	No of cycles
Plasmodium	F	TTAAAATTGTTGCAGTTAAAACG	1200 bp	95 °C, 1 min	53 °C, 0.75 min	72 °C, 1.5 min	35
Plasmodium	R	CCTGTTGTTGCCTTAAACTTC		95 °C, 1 min	53 °C, 0.75 min	72 °C, 1.5 min	35
P. falciparum	F	TTAAACTGGTTTGGGAAAACCAAATATATT	205 bp	95 °C, 1 min	58 °C, 0.75 min	72 °C, 1 min	35
P. falciparum	R	ACACAATGAACTCAATCATGACTACCCGTC		95 °C, 1 min	58 °C, 0.75 min	72 °C, 1 min	35
P. vivax	F	CGCTTCTAGCTTAATCCACATAACTGATAC	112 bp	95 °C, 1 min	60 °C, 1 min	72 °C, 1 min	35
P. vivax	R	ACTTCCAAGCCGAAGCAAAGAAAG TCCTTA		95 °C, 1 min	60 °C, 1 min	72 °C, 1 min	35
P. malariae	F	ATAACATAGTTGTACGTTAAGAATAACCGC	144 bp	95 °C, 1 min	58 °C, 0.75 min	72 °C, 1 min	35
P. malariae	R	AAAATTCCCATGCATAAAAAATTATACAAA		95 °C, 1 min	58 °C, 0.75 min	72 °C, 1 min	35
P. ovale	F	ATCTCTTTTGCTATTTTTTAGTATTGGAGA	800 bp	95 °C, 1 min	60 °C, 1 min	72 °C, 1 min	35
P. ovale	R	GGAAAAGGACACATTAATTGTATCCTAGTG		95 °C, 1 min	60 °C, 1 min	72 °C, 1 min	35

ISSN: 1475-2875