Fig. 3
Role of variant ATP2B4 genotype group on PMCA4b expression, function and P. falciparum erythrocytic stage growth. Individuals from homozygous (T/T) genotype (red), heterozygous (T/C) genotype (blue) and wild type (C/C) genotype (green) for the ATP2B4 variant haplotype A Expression of PMCA4b protein in RBCs from homozygous (T/T) donors (n = 29) compared to heterozygous (T/C) donors (n = 29) and wild type (C/C) donors (n = 30). ANOVA followed by Dunn’s multiple comparison testing was used (**** = p > 0.001). B Comparison of calcium expulsion from RBCs indicated by calcium half-life in RBCs from homozygous (T/T), (n = 13), heterozygous (T/C) donors (n = 13) and wild type (C/C) donors (n = 13). ANOVA followed by Dunn’s multiple comparison testing was used (**** = p > 0.001). C Inhibition of parasite growth by PMCA4b inhibitor aurintricarboxylic acid (ATA) in RBCs (n = 3 from wild type (C/C) donors). Inhibitory concentration at 50% (IC50) was calculated using a non-linear regression dose–response model. D Growth of P. falciparum strain FCR3-FMG in vitro in RBCs from homozygous (T/T) donors (n = 27), heterozygous (T/C) donors (n = 35) and wild type (C/C) (n = 30) E Growth of P. falciparum field isolates in vitro in RBCs from homozygous (T/T) donors (n = 3), heterozygous (T/C) donors (n = 3) and wild type (C/C) donors (n = 3) were infected with P. falciparum clinical strain 998. F Growth of P. falciparum field isolates in vitro in RBCs from homozygous (T/T) donors (n = 5), heterozygous (T/C) donors (n = 9) and wild type (C/C) donors (n = 5) were infected with P. falciparum clinical strain 952. Values for all panels (D) (E) and (F) are presented relative to growth in RBCs from wild type (C/C) donors ANOVA (*p < 0.5). Each dot represents the mean result of triplicate growth assays from each donor and the error bars represent SEM. G P. falciparum strain FCR3-FMG invasion in RBCs from homozygous (T/T) donors (n = 12), heterozygous (T/C) donors (n = 15) and wild type (C/C) donors (n = 13), Means were compared using one-way ANOVA (ns)