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Table 1 Primers, Thermal cycling conditions of the PCR and controls for msp1 and msp2

From: Low genetic diversity of Plasmodium falciparum merozoite surface protein 1 and 2 and multiplicity of infections in western Ethiopia following effective malaria interventions

Gene

PCR round

Primer name

Sequence (5'–3')

Cycling conditions

Positive control

msp1

Primary

N1-Fw

GCAGTATTGACAGGTTATGG

Initial denaturation: 94 °C for 5 min; PCR: 30 cycles of 94 °C for 30 s, 45 °C for 45 s, 70 °C for 1.5 min:; final elongation: 70 °C for 10 min

3D7, K1, HB3 and Dd2

N1-Re

GATTGAAAGGTATTTGAC

Nested

K1- Fw

ROX/AATGAAGAAGAAATTACTACAAAAGGTGC

Initial denaturation: 94 °C for 5 min; PCR: 30 cycles of 94 °C for 30 s, 52 °C for 45 s, 70 °C for 1.5 min; final elongation: 70 °C for 10 min

3D7

K1-Re

GTGTCTTGCTTGCATCAGCTGGAGGGCTTGCACCAG

MAD-Fw

6FAM/AAATGAAGGAACAAGTGGAACAGCTGTTAC

Dd2

MAD-Re

GTGTCTTATCTGAAGGATTTGTACGTCTTGAATTACC

RO33-Fw

HEX/TAAAGGATGGAGCAAATACTCAAGTTGTTG

7G8

RO33-Re

GTGTCTTCAAGTAATTTTGAACTCTATGTTTTAAATC

msp2

Primary

S2-Fw

GAAGGTAATTAAAACATTGTC

Initial denaturation: 94 °C for 5 min; PCR: 30 cycles of 94 °C for 30 s, 45 °C for 45 s, 70 °C for 1.5 min:; final elongation:70 °C for 10 min

3D7, K1 and HB3

S2-Re

GAGGGATGTTGCTGCTGCTCCACAG

Nested

Fc 27- Fw

6FAM/GCATTGCCAGAACTTGAA

Initial denaturation: 94 °C for 5 min; PCR: 30 cycles of 94 °C for 30 s, 52 °C for 45 s, 70 °C for 1.5 min; final elongation: 70 °C for 10 min

Dd2

3D7-fw

HEX/CTGAAGAGGTACTGGTAGA

3D7

Stail Re

GTGTCTTGCTTATAATATGAGTATAAGGAGAA

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