Table 1 Details of the primers, restriction enzymes and cycling conditions used for Pfcrt and Pfmdr1 RFLP analysis
Gene | Primer sequence (5'-3') | Product size (bp) & Restriction enzyme | PCR cycling conditions | |
|---|---|---|---|---|
Pfcrt-F | GGCTCCACGTTTAGGTGGA | 264 | CQS-128 & 136 bp | Initial: 95 ℃-5 min 30 cycles 95 ℃-1 min 48 ℃-45 s 72 ℃-2 min Final: 72 ℃-10 min |
Pfcrt-R | TGAATTTCCCTTTTTATTTCCAAA | Apo I | CQR-Undigested | Â |
Pfmdr1-F | ATGGGTAAAGAGCAGAAAGA | 603 bp | CQR-253 & 350 bp | Initial: 95 ℃-5 min, 30 cycles 95 ℃-1 min 56 ℃-45 s 72 ℃-2 min Final: 72 ℃-10 min |
Pfmdr1-R | AACGCAAGTAATACATAAAGTCA | Afl III | CQS-Undigested | Â |