Fig. 3
Substantially altered metabolite abundances in KAE609-treated and PA21A092-treated mutant parasite cultures. A FC40/8 values of metabolites detected in KAE609-treated cultures of mutant parasites compared with untreated cultures of wildtype parasites. B FC40/8 values of metabolites detected in KAE609-treated cultures of mutant parasites compared with PA21A092-treated cultures of mutant parasites. C FC40/8 values of metabolites detected in PA21A092-treated cultures of mutant parasites compared with untreated cultures of wildtype parasites. Here, FC40/8 denotes average fold change in abundance of a metabolite at the 40 h time point relative to its abundance at the 8 h time point. The average is based on 10,000 bootstrap calculations (see Methods). Red markers denote metabolites having fourfold different FC40/8 after treatment as compared to untreated conditions, while grey markers denote metabolites having less than twofold difference; all other metabolites are shown using green circles. Open red and green markers denote metabolites that tend to vary substantially over time in uninfected erythrocytes [49], while markers that have been filled black denote metabolites with no variability information. 5′-GMP 5′ guanosine monophosphate, AMP adenosine monophosphate, GSH reduced glutathione, KA09 KAE609 or cipargamin, MT-RBC erythrocytes infected with mutant parasites, NAD+ nicotinamide adenine dinucleotide, PA92 pyrazoleamide compound PA21A092, WT-RBC erythrocytes infected with wildtype parasites