Metabolic responses in blood-stage malaria parasites associated with increased and decreased sensitivity to PfATP4 inhibitors

Table 3 Macromolecules and metabolites accumulated in KAE609- and PA21A092-treated parasites

Macromolecule	\(\Delta {\text{m}}{{^a}} = \frac{\int {\text{v}}_{\text{PA92}}^{\text{t}}}{\int {\text{v}}_{\text{CTL}}^{\text{t}}}\)	\(\Delta {\text{m}}{{^a}}\text{ } = \frac{\int {\text{v}}_{\text{KA09}}^{\text{t}}}{\int {\text{v}}_{\text{CTL}}^{\text{t}}}\)
DNA	1.10	1.19
RNA	1.07	0.94
Protein	1.30	1.06
Phospholipid	1.59	0.99

Metabolite	\(\Delta {\text{m}}\)	\(\Delta {\text{m}}\)
Ammonium	3.25	1.76
S-adenosyl-L-methionine	2.56	1.29
Nicotinamide-adenine-dinucleotide	1.88	1.04
Protoheme	1.83	0.50
10-formyltetrahydrofolate	1.66	0.98
5–10-methylenetetrahydrofolate	1.44	0.87
Pyridoxal-5-phosphate	1.32	1.15
Coenzyme-A	1.31	1.00
Putrescine	1.26	1.27
Spermidine	1.25	0.79

^afold change in net synthesized amount of a macromolecule (or metabolite). Here, \(\int {\text{v}}_{\text{X}}^{\text{t}}\) denotes total accumulated amount of a macromolecule (or metabolite), computed numerically by integrating its synthesis rate (v) over the 48 h intraerythrocytic developmental cycle. Where, X is equal to untreated (CTL), KAE609-treated (KA09), or PA21A092 treated (PA92) conditions

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