Table 2 Genetic diversity of Pfdhfr and Pfdhps and the combined Pfdhfr/dhps haplotypes in P. falciparum isolates from Ile-Ife, Southwest Nigeria
Gene | Category of mutation | Haplotype | Community, n (%) | Pregnant, n (%) | Total, n (%) |
|---|---|---|---|---|---|
Pfdhfr (n = 74) | Wild type | CNCSI | 0 (0.0) | 0 (0.0) | 0 (0.0) |
Double | CNRNI | 1 (1.7) | 0 (0.0) | 1 (1.4) | |
CICNI | 1 (1.7) | 0 (0.0) | 1 (1.4) | ||
Triple | CIRNI | 55 (94.9) | 16 (100.0) | 71 (95.8) | |
Pfdhps (n = 61) | Wild type | ISAKAA | 0 (0.0) | 0 (0.0) | 0 (0.0) |
Single | ISGKAA | 12 (27.9) | 8 (44.4) | 20 (32.8) | |
IAGKAA | 2 (4.7) | 1 (5.6) | 3 (5.8) | ||
Double | IAGKAS | 2 (4.7) | 2 (11.1) | 4 (6.6) | |
VAGKAA | 5 (11.6) | 4 (22.2) | 5 (9.62) | ||
Triple | ISGKGS | 5 (11.6) | 0 (0.0) | 9 (14.8) | |
IAGKGS | 1 (2.3) | 0 (0.0) | 1 (1.6) | ||
VAGKAS | 5 (11.6) | 1 (5.6) | 6 (9.8) | ||
Quardruple | VAGKGS | 8 (18.6) | 1 (5.6) | 9 (14.8) | |
Pfdhfr/Pfdhps (n = 56) | Quadruple mutant | IRNI + ISGKAA | 11 (28.2) | 8 (47.1) | 19 (33.9) |
IRNI + IAGKAA | 2 (5.1) | 1 (5.9) | 3 (5.4) | ||
NRNI + VAGKAA | 1 (2.6) | 0 (0.0) | 1 (1.8) | ||
Quintuple mutant | ICNI + VAGKAS | 1 (2.6) | 0 (0.0) | 1(1.8) | |
IRNI + IAGKAS | 2 (5.1) | 2 (11.8) | 4 (7.2) | ||
IRNI + VAGKAA | 4 (10.3) | 4 (23.5) | 8 (14.2) | ||
Sextuple mutant | IRNI + ISGKGS | 5 (12.8) | 0 (0.0) | 5 (8.9) | |
IRNI + IAGKGS | 1 (2.6) | 0 (0.0) | 1 (1.8) | ||
IRNI + VAGKAS | 4 (10.3) | 1 (5.9) | 5 (8.9) | ||
Septuple mutant | IRNI + VAGKGS | 8 (20.5) | 1 (5.9) | 9 (16.1) |