Table 2 The number of specimens per species analysed by Sanger sequencing, amplicon sizes, and restriction patterns
Species | Number of specimens | ITS2 amplicon size (bp) | Predicted fragments sizes (bp) | ||
|---|---|---|---|---|---|
AluI/MseI | AluI | DdeI | |||
An. (Nyss.) albimanus | 76 | 561 | 268, 162, 91, 33, 7 | 366, 162, 33 | 370, 112, 85 |
An. (Nyss.) darlingi | 8 | 592 | 197, 172, 106, 77, 33, 7 | 303, 172, 84, 33 | 392, 112, 95 |
An. (Nyss.) argyritarsis | 2 | 526 | 240, 157, 89, 33, 7 | 336, 157, 33 | 417, 112 |
An. (Nyss.) albitarsis | 2* | 536 | 242, 143, 85, 33, 26, 7 | 267, 150, 84, 33 | 431, 108 |
An. (An.) vestitipennis | 14 | 583 | 247, 147, 80, 69, 33, 7 | 253, 216, 80, 33 | 317, 270 |
An. (An.) pseudopunctipennis | 7 | 565 | 525, 33, 7 | 528, 33 | 301, 150, 117 |
An. (An.) punctimacula | 2 | 445 | 216, 77, 71, 47, 33, 7 | 336, 76, 33 | 445 |
An. (An.) crucians | 13 | 388 | 213, 80, 55, 33, 7 | 213, 80, 62, 33 | 228, 164 |
An. (An.) apicimacula | 1 | 533 | 228, 120, 80, 42, 34, 7 | 372, 80, 47, 34 | 408, 118, 13 |
An. (An.) neomaculipalpus | 3 | 504 | 248, 120, 80, 33, 16, 7 | 255, 136, 80, 33 | 504 |
An. (An.) gabaldoni | 2 | 532 | 272, 140, 80, 33, 7 | 419, 80, 33 | 532 |
An. (Kert.) neivai | 2 | 583 | 247, 147, 80, 69, 33, 7 | 254, 216, 80, 33 | 316, 270 |