Laboratory evaluation of the miniature direct-on-blood PCR nucleic acid lateral flow immunoassay (mini-dbPCR-NALFIA), a simplified molecular diagnostic test for Plasmodium

Table 2 Results of the mini-dbPCR-NALFIA for repeated measurements of a FCR3 P. falciparum culture dilution in blood, in order to determine the limit of detection

Samples	No. of repetitions	Frequency of mini-dbPCR-NALFIA result per parasite density
Samples	No. of repetitions	Negative	Only positive for pan-Plasmodium line	Only positive for P. falciparum line	Positive for pan-Plasmodium and P. falciparum lines
P. falciparum parasites in blood
1000 p/µL	23	0	0	0	23 (100%)
100 p/µL	23	0	0	0	23 (100%)
10 p/µL	23	2 (9%)	0	7 (30%)	14 (61%)
5 p/µL	23	15 (65%)	0	7 (30%)	1 (4%)
2 p/µL	23	18 (78%)	0	5 (22%)	0
1 p/µL	23	19 (83%)	0	4 (17%)	0
0.1 p/µL	23	23 (100%)	0	0	0

ISSN: 1475-2875