Table 4 Results of the mini-dbPCR-NALFIA for a sample set tested by five different operators
Samples | No. of repetitions | Frequency of mini-dbPCR-NALFIA result | |||
|---|---|---|---|---|---|
Negative | Only positive for pan-Plasmodium line | Only positive for P. falciparum line | Positive for pan-Plasmodium and P. falciparum lines | ||
P. falciparum parasites in blood | |||||
100,000 p/µL | 5 | 0 | 0 | 0 | 5 |
10,000 p/µL | 5 | 0 | 0 | 0 | 5 |
1000 p/µL | 5 | 0 | 0 | 0 | 5 |
100 p/µL | 5 | 0 | 0 | 0 | 5 |
10 p/µL | 5 | 1 | 0 | 1 | 3 |
5 p/µL | 5 | 3 | 0 | 1 | 1 |
2 p/µL | 5 | 3 | 0 | 1 | 1 |
1 p/µL | 5 | 2 | 0 | 3 | 0 |
0.1 p/µL | 5 | 5 | 0 | 0 | 0 |
Blood from Dutch healthy donors | |||||
Blood donor 1 | 5 | 5 | 0 | 0 | 0 |
Blood donor 2 | 5 | 5 | 0 | 0 | 0 |
Blood donor 3 | 5 | 5 | 0 | 0 | 0 |
Blood donor 4 | 5 | 5 | 0 | 0 | 0 |
Blood donor 5 | 5 | 5 | 0 | 0 | 0 |