Table 2 Showing primers and gene fragment for evaluating artemisinin resistant P. falciparum markers
Gene fragment | PCR primer sequence | Mutations analyzed |
|---|---|---|
k13-propeller (1st round PCR) | 5′_CGGAGTGACCAAATCTGGGA-3 5′_GGGAATCTGGTGGTAACAGC-3 | T474I, M476I, A481V, Y493H, T508N, P527T, G533S, N537I, R539T, I543T, P553L, R561H, V568G, P574L, A578S, and C580Y |
k13-propeller (2nd round PCR) | 5′_TCAACAATGCTGGCGTATGTG-3 5′_TGATTAAG GTAATTAAAAGCTGCTCC-3 | T474I, M476I, A481V, Y493H, T508N, P527T, G533S, N537I, R539T, I543T, P553L, R561H, V568G, P574L, A578S, and C580Y |