Table 2 The expected fragment sizes after PCR–RFLP for the analysis of mutations at codons 976 and 1076 of the pvmdr1 gene
Position | Enzyme | Restriction site | Product size (bp) | Incubation °C/(h) |
|---|---|---|---|---|
Y976F | MboII | ↓AATT | Y: 154, 150, 150, 14 | 37 (4) |
F: 154, 150, 81, 69, 14 | Â | |||
F1076L | TasI (TspEI) | GAAGA(N)8/7↓ | F: 151, 124, 92, 43, 34 | 65 (1) |
L: 151, 124, 92, 77 | Â |