Malaria Journal BioMed Central

Background Plasmodium vivax is the second most prevalent malaria parasite affecting more than 75 million people each year, mostly in South America and Asia. In addition to major morbidity this parasite is associated with relapses and a reduction in birthweight. The emergence and spread of drug resistance in Plasmodium falciparum is a major factor in the resurgence of this parasite. P. vivax resistance to drugs has more recently emerged and monitoring the situation would be helped, as for P. falciparum, by molecular methods that can be used to characterize parasites in field studies and drug efficacy trials. Methods Practical PCR genotyping protocols based on polymorphic loci present in two P. vivax genetic markers, Pvcs and Pvmsp1, were developed. The methodology was evaluated using 100 P. vivax isolates collected in Thailand. Results and Discussion Analysis revealed that P. vivax populations in Thailand are highly diverse genetically, with mixed genotype infections found in 26 % of the samples (average multiplicity of infection = 1.29). A large number of distinguishable alleles were found for the two markers, 23 for Pvcs and 36 for Pvmsp1. These were generally randomly distributed amongst the isolates. A total of 68 distinct genotypes could be enumerated in the 74 isolates with a multiplicity of infection of 1. Conclusion These results indicate that the genotyping protocols presented can be useful in the assessment of in vivo drug efficacy clinical trials conducted in endemic areas and for epidemiological studies of P. vivax infections.


Background
Chloroquine (CQ) and sulfadoxine-pyrimethamine (SP) are the most frequently used antimalarial drugs in sub-Saharan Africa. With the spread and intensification of drug resistance of Plasmodium falciparum more effective combinatory drug regimes are now introduced in many African countries [1]. However, CQ and SP are cheap and widely available and will most likely be in use for some more years, particularly in home treatment [2][3][4].
In several East African countries where SP was introduced in response to intense CQ-resistance, the drug has gradually lost efficacy although the pace of this development is subject to controversy [5,6]. Previously, the core mutations linked with resistance to CQ and SP were found to be associated with each other in isolates from southern Ghana [7]. Although CQ and SP are structurally unrelated and the mutations conferring resistance are located on separate chromosomes [8][9][10], this finding suggests that parasites resistant to CQ may acquire resistance to SP more easily than sensitive ones, or vice versa. Studies in murine models [11] and in vitro [12,13] support this hypothesis. If this was true, the spread of CQ resistance could pave the way for an accelerated development of SP resistance, and thus, bear substantial importance to the health systems of affected regions. In addition, transmission potential may be increased in both SP and CQ resistant parasites [14,15].
Recently, mutations in the P. falciparum dihydrofolate reductase (dhfr) gene in northern Ghana were observed not only to be predictive for SP treatment failure but also to be associated with increased pre-treatment gametocyte carriage [16]. Here, associations between dhfr alleles, the core mutation in the P. falciparum chloroquine resistance transporter gene (crt T76), residual antimalarials, and gametocyte carriage in children with uncomplicated malaria were re-examined.

Methods
P. falciparum isolates were collected from children with uncomplicated malaria participating in a treatment trial in Tamale, Northern Region, Ghana, at the end of the rainy season 2002. The results of this trial and on the dhfr and dihydropteroate synthetase gene (dhps) patterns are described elsewhere [16,17]. In the present report, data from all 126 children with complete follow-up after SP treatment for whom dhfr and dhps were genotyped are analysed. The study protocol was approved by the Ethics Committee, Ministry of Health, Northern Region, and by the Health Research Unit, Ministry of Health, Accra, and parents' informed consent was obtained.
Venous blood was collected into EDTA. Asexual parasites and gametocytes were counted per ≥ 200 and 500 white blood cells, respectively, on Giemsa-stained thick blood films, and densities were calculated based on a putative mean WBC count of 8,000/µL. Gametocyte counts one week following treatment were available for 104 children. Pre-treatment levels of CQ and pyrimethamine in blood were measured by ELISA assays [21] with limits of detection of 10 ng/mL and 25 ng/mL, respectively. P. falciparum dhfr, dhps, and crt alleles were assessed by restriction fragment length polymorphisms (RFLP) of amplicons generated by nested PCR assays applying hot start procedures (HotStart Taq, Qiagen, Germany). Primers and conditions are described elsewhere [22,23] as are restriction enzymes and RFLP conditions to characterize the codons dhfr, 16, 51, 59, 108, 164; dhps, 436, 437, 540, 581, 613; and crt, 76. Laboratory strains were used as controls for PCR and RFLP assays. Electrophoresis was perfomed on 3% GTG agarose (FMC Bioproducts, USA) gels.
Frequencies were compared by χ 2 -test or Fisher's exact test, and continuous variables by Student's t-test, Mann-Whitney U-test, or Kruskal-Wallis test as applicable. Logistic regression models were used to adjust for potential confounders of the presence of resistance mutations and to identify risk factors for gametocytaemia.
Dhfr, dhps, and crt alleles were examined with respect to pre-treatment gametocytaemia. As reported elsewhere [16], children were significantly more likely to harbour gametocytes in the presence of dhfr 51, 59, or triple mutations. This was also true for isolates exhibiting crt T76 (Tab. 2). Gametocytes were not observed in any (0/21) isolate with wildtype alleles for both dhfr and crt, but in 16% (8/50) of isolates exhibiting either dhfr or crt mutations, and in 29% (16/55) of isolates revealing both, dhfr triple and crt T76 (χ 2 trend = 8.7, P = 0.003). Adjusting for additional factors influencing gametocytaemia, i.e. high parasite density arbitrarily set as >50,000/µL, the presence of CQ in blood, and axillary temperature, both the dhfr triple mutation and crt T76 were associated with an increased likelihood of gametocytaemia (Tab. 2). Dhfr alleles or crt T76 were not associated with gametocyte density (data not shown).
Finally, selection of crt T76 in SP treatment failures was examined. No selection was observed: In matched pairs of isolates obtained from 35 children suffering SP treatment failure, crt T76 was present in 86% (30/35) of pre-treatment isolates and in 80% (28/35) of isolates obtained at treatment failure.

Discussion
In this study on P. falciparum isolates from northern Ghana, two major findings are presented. First, SP and CQ resistance markers are strongly associated with each other, independent of residual antimalarials. Second, both are associated with an increased prevalence of gametocytes.
This study has several limitations and particularly the finding of an association between unrelated mutations needs caution in interpretation. Because polyclonal infections predominate in the area [24], the detection of a mutant allele in an isolate does not necessarily mean that all clones carry the mutation. Thus, it cannot be stated whether the linkage between the resistance markers is a true one, i.e. on the chromosomal level, or reflects cooccurrence in individual isolates. The limited number of crt wildtypes also hampered proper testing of a linkage disequilibrium. Although dhfr mutations were significantly more common in the presence of crt T76 this observation needs to be construed with caution since 80% of the isolates in this study harboured the latter variant. Also, it is impossible to comment whether the presence of crt T76 favours the presence of dhfr mutations or vice versa because this requires longitudinal observations. Likewise, the lack of temporal information impairs a clear statement on whether resistance mutations bring about increased gametocytogenesis. Due to methodology the resistance genotype of asexual parasites cannot be separated from that of gametocytes.
Despite these difficulties in drawing firm conclusions, several previous findings support the hypothesis of a linkage disequilibrium between mutations associated with SP resistance and CQ resistance. Early reports from the 1950s and 1960s indicated that in areas or patients with established pyrimethamine-resistance in Nigeria, Burkina Faso, and Venezuela, CQ exhibited a reduced activity (reviewed in [25]). In a murine malaria model, CQ resistance could be induced in pyrimethamine-resistant parasites but not in sensitive ones [11]. In isolates from Cameroon, the in vitro activity of pyrimethamine was ten times lower in CQ resistant than in sensitive P. falciparum [13]. Similar but less pronounced differences have also been observed in other studies [26,27]. In southern Ghana, we previously observed that the dhfr core mutation Asn-108 was three times more likely found in isolates exhibiting crt T76 than in isolates comprising crt wildtype parasites [7]. The reason for this apparent association between resistance to CQ and pyrimethamine or SP is obscure since both drugs have distinct modes of action and resistance to these is determined by mutations on different chromosomes [8][9][10]. One alluringly simple explanation could be that parasites in the study area have merely become resistant to both drugs, possibly as a result of drug pressure. In this regard, previous, simultaneous or sequential treatment with CQ and SP could have selected for resistant parasites which subsequently persisted for a longer period than the drugs can be detected in blood. However, pyrimethamine was seen in only two children which indicates that it is rarely used in this community and which argues against drug-induced selection for SP resistance. In addition, the results are corrected for the presence of CQ in blood which can be detected for approximately one month after intake [28]. This does not exclude the possibility of selected and persisting parasites but renders it rather unlikely. Alternatively, the association between crt T76 and dhfr mutations could reflect a rapid mutator phenotype with the ability of accelerated resistance to multiple drugs. This hypothesis originates from in vitro studies observing that parasites resistant to common antimalarials acquire resistance to structurally unrelated drugs more rapidly than susceptible strains. The genetic basis of this phenomenon is unknown but suggested to involve an increased frequency of mutations per se and consequently a higher probability of modified proteins which could also include drug targets [12]. In fact, selection for highgrade pyrimethamine resistance in vitro has been shown to enhance the degree of overall genomic polymorphism [29]. In this regard, it is noteworthy that crt T76 was more frequently observed with increasing number of dhfr mutations, i.e. with increasing degree of SP resistance.
In the present study, the association between the resistance markers meets with an increased presence of gametocytes in isolates comprising mutant dhfr or crt alleles. Gametocytaemia seemed to reflect a rather long duration of infection as can be deduced from its low prevalence in the presence of factors suggestive for acute disease, i.e. high body temperature and parasite density, and previous CQ treatment (Tab. 2). Again, an increased frequency of resistance mutations in gametocytaemic children could result from previous drug-related selection as outlined above. However, increased gametocytaemia preceding treatment has also been observed in infections subsequently found to be CQ resistant [30][31][32]. Hallett et al. [15] reported that in patients with crt T76 parasites, gametocyte density was highly increased one week following CQ treatment. In addition, gametocytes from patients carrying crt T76 parasites produced 38 times higher oocyst burdens in the mosquito as compared to crt wildtype parasites. In Tamale, both residual CQ levels and parasites with the crt T76 mutation are abundant [33], and a clear association of crt T76 and pre-treatment gametocyte prevalence is seen. Only one crt wildtype isolate contained gametocytes impairing a sound analysis of the effect of crt T76 on gametocyte density. However, increased gametocyte production by crt T76 parasites [15] in the presence of residual CQ could partially explain the present finding. The synthesis of both, crt and dhfr mutations being associated and increased gametocytaemia in their presence, gives rise to a grim scenario: Given that CQ resistant parasites have an improved transmission potential [15] the association with SP resistance would contribute to an accelerating spread of resistance to both drugs, particularly in areas where CQ resistance is frequent. In the present study, neither selection of crt T76 in SP treatment failure was observed nor a significantly elevated proportion of post-treatment gametocytaemia among children initially harbouring crt T76 parasites. Although this was not expected and would reflect an extraordinary rapid process, both observations may be influenced by the small sample size.

Conclusion
The present data provide evidence supporting a hypothesis on a connection between resistance to CQ and SP suggesting that both, CQ and SP resistance favour transmission. This needs to be verified by carefully designed longitudinal studies in regions of differing levels of drug resistance and endemicity. Per se, antimalarial treatment must be effective, and more effective than CQ and SP, not only to reduce treatment failures but also the transmission of potential co-resistance. Eventually, as this has been shown to counterbalance enhanced transmission of resistant parasites [15] the results strongly support combinatory treatment including artemisinine-derivatives.

Authors' contributions
FPM, JTB, and UB designed the study. RNO, SE and FPM were responsible for patient recruitment, clinical and parasitological examinations, and PCR assays. TAE measured drug concentrations. JTB and RWS did the gametocyte counts. FPM and JTB wrote the paper with major contributions of the other authors.